Objective: To investigate the
protective effect of recombinant human IL-11 (rhIL-11) administered in a
systemic manner on retina tissue during ischemia/reperfusion (I/R) injury in a
guinea pig model.
Study design/materials and methods:
An experimental study in retinal I/R. Placebo, ischemia/sham, ischemia/ rhIL-11
groups including five animals in each were formed from male albino guinea pigs.
Retinal ischemia was induced by cannulating the anterior chambers and lifting
the bottle to a height of 205 cm for 90 Min in the sham and ischemia/rhIL-11
groups. The ischemia/sham and ischemia/rhIL-11 groups received 0.1 cc of asaline solution and 5 μg/kg/day rhIL-11 intraperitoneally one hour before the
ischemic insult and during two days of reperfusion, respectively. The guinea
pigs were sacrificed for biochemical analysis and the levels of tumor necrosis
factor alpha (TNF-α), transforming growth factor beta (TGF-β) and interleukin-6
(IL-6) in retina were analyzed with ELISA. Mann–Whitney-U and Kruskal–Wallis
tests were used for statistical analysis.
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