Though keratoplasty is used to
treat corneal blindness, donor shortage, tendency of stimulated keratocyte
transformed to fibroblast and immunological rejection are still big problems.
As a solution, cornea tissue engineering using non-corneal tissue sourced cells become emerging issue. Thus, this study was designed to find novel material for
keratoplasty.
Human turbinate-derived mesenchymal stem cells (hTMSCs)
were obtained from patients and cultured with differentiation medium for 14
days. The keratocyte markers, stem cell markers, early corneal stromal stem cell (CSSC) markers, were measured by real time-PCR. The MSC markers were
detected by FACS. Results: After 14 days of differentiation medium exposure,
hTMSCs expressed markers of keratocyte such as keratocan sulfate proteoglycan
(KERA) and aldehydrogenase (ALDH). As the hTMSCs became keratocytes, the
expression of embryonic ocular precursor markers ABCG2 and PAX6 decreased but
were still measurable. Early CSSC markers including SIX2, SIX3, BMI expression
was elevated after 7 d and reduced after 14 d of KDM treatment.
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